Application

Anti-Nitric Oxide Synthase III Antibody detects level of Nitric Oxide Synthase III & has been published & validated for use in WB & IC.

Research CategoryNeuroscience

Western Blot: 5 µg/mL using chemiluminescent detection. Detects ~135-140kDa band in endothelial cell preparations; Higher concentrations of the antibody may be required if colorimetric detection is used. Antibody dilution will also vary with the concentration of the antigen. Immunocytochemistry: Use at 5-10 µg/mL

Optimal working dilutions must be determined by end user.

Research Sub CategoryOxidative Stress

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide corresponding to amino acids 596-610 of human endothelial NOS, coupled to KLH.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% Sodium azide as a preservative.

Specificity

Reactive with human NOS-III (eNOS). This immunogen sequence is 100% homologous in human, bovine, rat, and mouse, but reactivity has not been tested.

Storage and Stability

Store at 2-8°C in undiluted aliquots for up to 6 months.